什么是ELISA 稀釋劑？一文了解概述、類型和應用

<p>&nbsp;<font face="宋體" style="font-family: 宋體; font-size: 10.5pt;">　　酶聯免疫吸附試驗</font><font face="Calibri" style="font-size: 10.5pt;">(ELISA)</font><font face="宋體" style="font-family: 宋體; font-size: 10.5pt;">是一種常用的基于微孔板（例如</font><font face="Calibri" style="font-size: 10.5pt;">96</font><font face="宋體" style="font-family: 宋體; font-size: 10.5pt;">孔板）的檢測方法，廣泛應用于免疫學和生物化學實驗室。它利用抗原抗體反應的原理，可以定性和定量分析各種生物分子和化學物質，例如肽、蛋白質、抗體、激素、小分子、藥物和毒素等。</font></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">利用一系列試劑來檢測目標物質。其中最關鍵的試劑包括包被抗原</font><font face="Calibri">/</font><font face="宋體">抗體、檢測抗體、底物和終止液。此外，還需要其他輔助試劑，如封閉液和洗滌液，以確保檢測的準確性和靈敏度。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">中常用的稀釋劑包括樣品稀釋劑和測定稀釋劑（或抗體稀釋劑）。樣品稀釋劑用于稀釋待測樣品，而測定稀釋劑用于稀釋檢測抗體。兩者都有助于降低背景噪音，提高檢測的準確性。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　稀釋劑如何在測定中發揮作用并與其他成分相互作用以提供結果？哪些工作流程涉及使用稀釋劑？哪些行業經常使用它們？下面由酶聯生物詳細為您解答。</span></p> <p class="MsoNormal">&nbsp;</p> <p class="MsoNormal" style="text-align: center;"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><img src="/images/upload/Image/10(23).png" width="500" height="375" alt="酶聯生物elisa試劑盒" /></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑有哪些作用？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">實驗中會用到多種試劑，包括包被緩沖液、封閉緩沖液、結合物穩定劑、底物、終止液以及各種稀釋劑。其中，稀釋劑主要包括樣品稀釋劑和抗體</font><font face="Calibri">/</font><font face="宋體">測定稀釋劑。樣品稀釋劑用于稀釋待測樣品，而抗體</font><font face="Calibri">/</font><font face="宋體">測定稀釋劑用于稀釋檢測抗體，并提供最佳的反應環境。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">還需要標準品和待測樣品。標準品是已知濃度的目標分析物，用于繪制標準曲線。待測樣品則包含未知濃度的目標分析物。由于樣品基質的復雜性，可能會對檢測結果造成干擾。為了減少基質效應，需要進行樣品預處理或選擇合適的樣品稀釋劑。樣品基質和標準品基質的差異應該通過標準品的選擇和樣品預處理來盡量減小，而不是依賴于檢測稀釋劑。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑的類型</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　在</font><font face="Calibri">ELISA</font><font face="宋體">實驗中經常用到</font><font face="Calibri">2</font><font face="宋體">種類型稀釋劑，包括樣品稀釋劑和測定稀釋劑</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　樣品稀釋液是間接</font><font face="Calibri">ELISA</font><font face="宋體">檢測的第一步中用于評估抗體和蛋白質的特定緩沖溶液。在免疫檢測（例如</font><font face="Calibri">ELISA</font><font face="宋體">和</font><font face="Calibri">WesternBlotting</font><font face="宋體">檢測）中，稀釋液有助于：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">盡量減少非特異性結合</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">降低背景噪音</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">減少樣本變異</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　市場上可用的樣品稀釋劑類型包括：</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>血漿樣本稀釋液</strong>：一種富含添加劑的</font><font face="Calibri">BSA</font><font face="宋體">緩沖液，可在</font><font face="Calibri">ELISA</font><font face="宋體">板孵育階段防止凝血（凝血酶抑制）和補體激活。它可用于血漿樣本的抗原向下</font><font face="Calibri">ELISA</font><font face="宋體">格式和血漿樣本的抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>通用血清稀釋劑</strong>：緩沖</font><font face="Calibri">BSA</font><font face="宋體">溶液，在特定抗原抗體相互作用測定過程中起到維持恒定溶質和</font><font face="Calibri">pH</font><font face="宋體">環境的作用。它們經常用于稀釋哺乳動物和雞血清樣本。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="Calibri">Neptune</font><font face="宋體">樣品稀釋劑</font></strong><font face="宋體">：一種非哺乳動物蛋白質溶液。它與血清和血漿樣品一起用于抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">檢測。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　<strong>樣品稀釋劑優化包</strong>：上述三種稀釋劑的混合物。用于確定哪種稀釋劑適合您的研究用途。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　抗體</font><font face="Calibri">/</font><font face="宋體">檢測抗體稀釋劑，主要用于稀釋</font><font face="Calibri">ELISA</font><font face="宋體">中的檢測抗體，并提供最佳的抗原抗體反應環境。它有助于提高檢測的靈敏度、特異性和穩定性。用途如下：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="宋體">解決抗原向下或抗體夾心</font><font face="Calibri">ELISA</font><font face="宋體">中的基質問題</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">減少樣品基質干擾</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">盡量減少非特異性相互作用</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">降低背景噪音</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　實驗室應用中使用的分析稀釋劑的類型包括：</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>通用檢測稀釋劑</strong>：抑制凝血和補體活性，廣泛用于夾心</font><font face="Calibri">ELISA</font><font face="宋體">檢測中的血漿或血清樣本。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>海王星測定稀釋劑</strong>：抑制凝血酶和補體活性。在抗原下降</font><font face="Calibri">ELISA</font><font face="宋體">中與血清和血漿樣本一起使用。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="Calibri">IgM</font><font face="宋體">還原測定稀釋劑</font></strong><font face="宋體">：通過抑制補體和凝血酶活性，同時最大限度地減少</font><font face="Calibri">IgM</font><font face="宋體">介導的結合橋接干擾來發揮作用。它在夾心</font><font face="Calibri">ELISA</font><font face="宋體">中與血漿和血清樣本一起使用。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>抗原降低測定稀釋劑</strong>：用于防止</font><font face="Calibri">ELISA</font><font face="宋體">微孔板中樣品凝結。適用于血漿和血清樣品的抗原降低</font><font face="Calibri">ELISA</font><font face="宋體">。</font><font face="Calibri">&nbsp;</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　<strong>分析稀釋劑優化包</strong>：包含上述所有四種稀釋劑，幫助您確定哪種稀釋劑最適合您的研究應用。</span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <h3><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　哪些行業使用</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑？</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></h3> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">檢測稀釋劑是一種緩沖溶液（通常是含有牛血清溶液的磷酸鹽緩沖鹽水</font><font face="Calibri">(PBS)</font><font face="宋體">），用于稀釋</font><font face="Calibri">ELISA</font><font face="宋體">樣品或均衡檢測基質。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　該緩沖液專為</font><font face="Calibri">ELISA</font><font face="宋體">應用而設計并驗證，包括：</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <ol> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="宋體">阻斷抗體包被的</font><font face="Calibri">ELISA</font><font face="宋體">板微孔</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><strong><font face="宋體">稀釋</font><font face="Calibri">ELISA</font><font face="宋體">標準品和樣品</font></strong></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></li> <li><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　<strong>稀釋生物素化的檢測抗體或三級試劑</strong>（例如</font><font face="Calibri">Avidin-HRP</font><font face="宋體">或</font><font face="Calibri">Streptavidin-HRP</font><font face="宋體">）。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></li> </ol> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">藥品</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑用于稀釋各種生物樣本，例如血液、血清和血漿。這可確保最佳濃度以有效結合和檢測目標分析物，從而提供準確可靠的檢測結果。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">　　</span><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">生物技術</span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　生物技術人員經常使用</font><font face="Calibri">ELISA</font><font face="宋體">檢測進行各種研究應用，例如分析蛋白質和抗體。這使得獲得準確的結果變得至關重要，而無需反復執行這些檢測。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　這是通過使用</font><font face="Calibri">ELISA</font><font face="宋體">稀釋劑來稀釋樣品、降低背景噪音并最大限度地減少</font><font face="Calibri">ELISA</font><font face="宋體">反應中不必要的相互作用來實現的。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;">&nbsp;</span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><font face="宋體">　　</font><font face="Calibri">ELISA </font><font face="宋體">稀釋劑，包括樣品稀釋劑和測定</font><font face="Calibri">/</font><font face="宋體">抗體稀釋劑，對于獲得準確可靠的 </font><font face="Calibri">ELISA </font><font face="宋體">結果至關重要。樣品稀釋劑用于處理樣品，而測定</font><font face="Calibri">/</font><font face="宋體">抗體稀釋劑用于稀釋檢測抗體并優化反應環境。選擇合適的稀釋劑需要考慮多種因素，并仔細查閱供應商提供的資料以及根據實驗需求進行驗證。 試劑盒制造商的推薦通常是不錯的選擇。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;&#10;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:10.5000pt;&#10;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>