ELISA試劑盒實驗的溫育方法

<p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">實驗中通常有兩次抗原抗體反響，即加標本和加酶聯絡物后。抗原抗體反響的完畢需求有必定的溫度和時刻，這一保溫進程稱為溫育</font><font face="Calibri">(incubation)</font><font face="宋體">，有人稱之為孵育，在</font><font face="Calibri">ELISA</font><font face="宋體">中似不恰當。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">屬固相免疫測定，抗原、抗體的聯絡只在固相表面上發生。以抗體包被的夾心法為例，參加板孔中的標本，其間的抗原并不是都有對等的和固相抗聯絡的時機，只需最貼近孔壁的一層溶液中的抗原直接與抗體觸摸。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">這是一個逐漸平衡的進程，因此需經渙散才調抵達反響的完畢。在這今后參加的酶符號抗體與固相抗原的聯絡也相同如此。這便是為何</font>ELISA<font face="宋體">反響老是需求必定時刻的溫育。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">溫育常選用的溫度有</font>43<font face="宋體">℃、</font><font face="Calibri">37</font><font face="宋體">℃、室溫文</font><font face="Calibri">4</font><font face="宋體">℃（冰箱溫度）等。</font><font face="Calibri">37</font><font face="宋體">℃是實驗室中常用的保溫溫度，也是大多數抗原抗體聯絡的合適溫度。在建立</font><font face="Calibri">ELISA</font><font face="宋體">方法作反響動力學研討時，實驗標明，兩次抗原抗體反響通常在</font><font face="Calibri">37</font><font face="宋體">℃經</font><font face="Calibri">1-2</font><font face="宋體">小時，產品的生成可達高峰。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">為加快反響，可前進反響的溫度，有些實驗在</font>43<font face="宋體">℃進行，但不宜選用更高的溫度。抗原抗體反響</font><font face="Calibri">4</font><font face="宋體">℃更為徹底，在放射免疫測定中多使反響在冰箱中過夜，以構成最多的堆積。但因所需時刻太長，在</font><font face="Calibri">ELISA</font><font face="宋體">中通常不予選用。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;">ELISA<font face="宋體">試劑盒</font></span></strong><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">保溫的方法除有的</font><font face="Calibri">ELISA</font><font face="宋體">儀器附有特制的電熱塊外，通常均選用水浴，可將</font><font face="Calibri">ELISA</font><font face="宋體">板置于水浴箱中，</font><font face="Calibri">ELISA</font><font face="宋體">板底應貼著水面，使溫度活絡平衡。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">為防止蒸發，板上應加蓋，也可用塑料貼封紙或保鮮膜覆蓋板孔，此時可讓反響板漂浮在水面上。若用保溫箱，</font>ELISA<font face="宋體">板應放在濕盒內，濕盒要選用傳熱性杰出的資料如金屬等，在盒底墊濕的紗布，最終將</font><font face="Calibri">ELISA</font><font face="宋體">板放在濕紗布上。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">濕盒應先放在保溫箱中預溫至規則的溫度，特別是在氣溫較低的時分更應如此。無論是水浴仍是濕盒溫育，反響板均不宜疊放，以確保各板的溫度都能活絡平衡。室溫溫育的反響，操作時的室溫應嚴重束縛在規則的范圍內，標準室溫溫度是指</font>20-25<font face="宋體">℃，但具體操作時可根據說明書的央求操控溫育。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p> <p class="MsoNormal"><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><font face="宋體">室溫溫育時，</font>ELISA<font face="宋體">板只需平置于操作臺上即可。應留神溫育的溫度和時刻應按規則力求精確。為確保這一點，一個人操作時，一次不宜多于兩塊板一同測定。</font></span><span style="mso-spacerun:'yes';font-family:宋體;mso-ascii-font-family:Calibri;mso-hansi-font-family:Calibri;mso-bidi-font-family:'Times New Roman';font-size:9.0000pt;mso-font-kerning:1.0000pt;"><o:p></o:p></span></p>